Compositions and methods for enhancing hybridization and priming specificity
US6361940B1 · kind B1 · utility
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16References
97Claims
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Key dates
| Filing date | Apr 1, 1998 |
| Grant date | Mar 26, 2002 |
| Priority date | — |
| Expiry date | Apr 1, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods are provided for increasing the specificity of a probe nucleic acid for a target nucleic acid in a hybridization solution. An abasic residue, deoxyNebularine residue, or a hybotrope is used to increase specificity. A method is provided for identifying useful hybotropes, including salts, water miscible organic solvents, aprotic solvents and organic solvents, on the basis of enthalpy considerations. Hybotropic hybridization and modified oligonucleotides may be used in amplification reactions, such as PCR, sequence analysis methods, and genomic screening methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.