Process for purifying hepatitis B viral surface antigen comprising pres2 peptide
US6362320B1 · kind B1 · utility
4Cited by
2References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 2, 1999 |
| Grant date | Mar 26, 2002 |
| Priority date | — |
| Expiry date | Nov 2, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2730/10122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for purifying hepatitis B viral surface antigen comprising the preS2 peptide from the cells of a recombinant organism is carried out by a sequence of steps which includes the step of disrupting the cells using a buffer containing a chaotropic salt to obtain a cell homogenate and the step of alkalifying the cell homogenate to a pH ranging from 11.0 to 13.5.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.