Conversion of a watson-crick DNA to a hoogsteen-paired duplex
US6362322B1 · kind B1 · utility
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Key dates
| Filing date | Jan 19, 2000 |
| Grant date | Mar 26, 2002 |
| Priority date | — |
| Expiry date | Jan 19, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/317
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method is described for obtaining Hoogsteen-paired pyrimidine*purine duplexes, either by heating a triplex to dissociate a Watson-Crick paired pyrimidine strand or by linking two parallel strands at their 5′ ends is disclosed. This duplex can be used as a new type of antisense molecule to pair with an RNA pyrimidine target sequence within an mRNA molecule. This duplex can also be used as a new type of antigene molecule to pair with a single-stranded DNA pyrimidine target sequence within the genome.
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