Assays and probes with enzyme labels
US6362328B1 · kind B1 · utility
4Cited by
5References
10Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 20, 1999 |
| Grant date | Mar 26, 2002 |
| Priority date | — |
| Expiry date | Sep 20, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/535
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Probes comprise S1 and P1 nuclease (as an enzyme label) linked to a specific binding member such as a nucleotide sequence or an antibody. Such probes are useful for sandwich assays. As compared with known probes using alkaline phosphatase as a label, advantages include relative insensitivity to phosphate and elevated temperature and reduced risk of nonspecific binding.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.