Methods and reagents for the rapid and efficient isolation of circulating cancer cells
US6365362B1 · kind B1 · utility
282Cited by
12References
15Claims
0Family size
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Key dates
| Filing date | Feb 12, 1999 |
| Grant date | Apr 2, 2002 |
| Priority date | — |
| Expiry date | Feb 12, 2019 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01F1/0054
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater sensitivity than conventional PCR or immunohistochemistry by 1-2 orders of magnitude. In addition, the assay facilitates the biological characterization and staging of carcinoma cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.