Process for removing protein aggregates and virus from a protein solution
US6365395B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 3, 2000 |
| Grant date | Apr 2, 2002 |
| Priority date | — |
| Expiry date | Nov 3, 2020 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D2311/04
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A process is provided for selectively removing protein aggregates and virus particles from a protein solution in a two-step filtration process. In a first step, a protein solution is filtered by tangential flow filtration through a cellulosic ultrafiltrate membrane at a transmembrane pressure of between about 1 and about 10 psi to produce a first permeate and a retentate stream. Water or aqueous buffer is added to the retentate stream to form an essentially constant volume retentate stream. The first permeate is filtered through a second ultrafiltration membrane to retain virus particles at a retention level of at least 3 LRV and to allow passage therethrough of a protein aggregate free and virus free protein solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.