High specificity primers, amplification methods and kits

US6365729B1 · kind B1 · utility

Assignee

• The Public Health Research Institute Of the City of New York, Inc. · US

Inventors

• Sanjay Tyagi · New York, US
• Fred R. Kramer · Yonkers, US
• Robert Vartikian · New York, US

Key dates

• Filing date: Jul 12, 2001
• Grant date: Apr 2, 2002
• Expiry date: Jul 12, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

For nucleic acid amplification including extension of primers by a DNA polymerase, high specificity primers are provided. The primers include a type of hairpin structure in which a single-stranded loop separates complementary 3′ and 5′ arms and in which the loop and the 3′ arm are complementary to the target nucleic acid. Amplification methods, assays and kits including such primers are included in the invention.