Electroporation buffer with cryprotective capabilities
US6368784B1 · kind B1 · utility
13Cited by
2References
20Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Oct 5, 1999 |
| Grant date | Apr 9, 2002 |
| Priority date | — |
| Expiry date | Oct 5, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The instant invention is a novel buffer which enables simultaneous cryoprotection and transfection of mammalian cells. It enables the user to make cell stocks which can be kept long term. Use of these cell stocks circumvents the need to culture cells each time a transfection is undertaken. It alleviates the need for continuous cell culture and repeated transfections due to transfection variability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.