E2F ubiquitination domain, and assays for inhibitors and enhancers of E2F ubiquitination
US6368809B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 23, 1999 |
| Grant date | Apr 9, 2002 |
| Priority date | — |
| Expiry date | Feb 23, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The transcription factor E2F contains a ubiquitination domain. Assays for inhibitors and enhancers of the domain are provided. An assay method for an inhibitor of transcription factor E2F ubiquitin-mediated degradation comprises a) bringing a polypeptide which contains a domain which renders E2F a substrate for ubiquitination into contact with a candidate inhibitor; and b) determining whether or not the candidate inibitor is capable of reducing ubiquitination of the polypeptide. An assay method for an enhancer of transcription factor E2F ubiquitin-mediated degradation may comprise a) bringing a polypeptide which contains a domain which renders E2F a substrate for ubiquitination into contact with a candidate enhancer; and b) determining whether or not the candidate enhancer is capable of enhancing ubiquitination of said polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.