Identification of salmonella
US6368817B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 8, 1999 |
| Grant date | Apr 9, 2002 |
| Priority date | — |
| Expiry date | Jul 8, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/924
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A new culture medium for identifying the presence of Salmonella inenterobacteria samples, especially faeces, contains two chromogenic enzyme substrates, one of which is a substrate for &agr;-D-galactosidase, for which Salmonella is positive. The other substrate is one for which Salmonella is negative such as &bgr;-D-galactosidase. The substrates are incorporated into an agar medium. Positive and negative results are found to be readily observable where one of the substrates is an esculetin, preferably a cyclohexenoesculetin compound in the presence of ferric ions, which produces a black color, and the other substrate is an indoxyl compound, for instance, a 5-bromo-4-chloro-3-indolyl compound which produces a green colored enzymic reaction product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.