Oligonucleotide mediated nucleic acid recombination

US6368861B1 · kind B1 · utility

Assignee

• Maxygen, Inc. · US

Inventors

• Andreas Crameri · Los Altos Hills, US
• Willem P. C. Stemmer · Los Gatos, US
• Jeremy Minshull · Los Altos, US
• Steven H. Bass · Hillsborough, US
• Mark Welch · Fremont, US
• Jon E. Ness · Sunnyvale, US
• Claes Gustafsson · Belmont, US
• Phillip A. Patten · Portola Valley, US

Key dates

• Filing date: Jan 18, 2000
• Grant date: Apr 9, 2002
• Expiry date: Jan 18, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16122
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.