Dyes and methods of reticulocyte enumeration
US6368864B1 · kind B1 · utility
11Cited by
17References
34Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 5, 2000 |
| Grant date | Apr 9, 2002 |
| Priority date | — |
| Expiry date | May 5, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/101666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A dye compound having the formula I: wherein,n is 0, 1, 2, or 3; R1 is H, alkyl, or an alkoxy group; R2 is CH2(CH2)mOH, wherein m is 0, 1, 2, or 3; X is O, S, or C(CH3)2; R is CH3, CH(CH3)2, CH2CH2OH, alkyl, alkylsulfonate, or hydroxyalkyl and B− is a counteranion.Red- and blue-excitable dyes based on the above chemical structure are described, and reagents containing them are described for use in staining nucleic acid, more particularly for staining reticulocytes. Also described are methods for detecting reticulocytes using such compositions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.