Apparatus for integrated polymerase chain reaction and capillary electrophoresis
US6372484B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 2000 |
| Grant date | Apr 16, 2002 |
| Priority date | — |
| Expiry date | Jan 21, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2547/101
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus is described for performing polymerase chain reaction (PCR) and capillary electrophoresis (CE) within a single, integrated and disposable device. Fluid enters the device via a sample charging port. From the sample charging port, the fluid travels through an inflow channel to the polymerase chain reaction chamber. This chamber is formed at a thin tab extension of a top portion of the device, and is generally defined by a cavity within the thin tab extension. After PCR is performed, the fluid leaves the polymerase chain reaction chamber through an outflow channel into a first sample well. A vent channel is connected to the first sample well for venting the first sample well to ambient atmosphere. The first sample well and a second sample well are in fluid communication by a sample channel. An electrophoresis separation channel is orthogonal to and intersects the sample channel, and has a first and second electrophoresis buffer well at each end. To perform electrophoresis, voltage is applied to the first and second sample well, and is then applied to the first and second electrophoresis buffer well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.