Polymeric complexes for the transfection of nucleic acids, with residues causing the destabilisation of cell membranes
US6372499B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 3, 1999 |
| Grant date | Apr 16, 2002 |
| Priority date | — |
| Expiry date | May 3, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P31/12
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The complex has at least one negatively charged nucleic acid bonded to at least one positively charged polymeric conjugateThe conjugate containing a polylysine formed from monomers having free NH3+ groups, and having at least 10% of the free NH3+ groups substituted by residues which can be protonated in a weakly acid medium causing destabilization of cell membranes.Optionally, some of the free NH3+ groups can be substituted by a molecule with a recognition signal by a cell membrane receptor.The free NH3+ groups of the said polylysine make up at least 30% of the monomers of the polymeric conjugate.The residue that causes the destabilization of cell membrane in weak acid of quinolines of the formula: where R1 is hydrogen, R2 is —(CH2)n13 CO2—H, X is hydrogen or chlorine and n is an integer from 1 to 10.The signal is a simple oside or a disaccharide or peptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.