Retroviral packaging cell lines and processes of using same

US6372502B1 · kind B1 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Arthur Bank · Yonkers, US
• Dina G. Markowitz · New York, US
• Stephen P. Goff · Orefield, US

Key dates

• Filing date: Nov 17, 1994
• Grant date: Apr 16, 2002
• Expiry date: Nov 17, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/13052
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a mammalian cell useful for retroviral packaging comprising two plasmids, both of which comprise the 5′ long terminal repeat (LTR) sequence from a helper virus, neither of which comprise a functional &psgr; packaging sequence or a 3′ LTR from the helper virus, one of which comprises the env gene from the helper virus and the other of which comprises the gag and pol genes from the helper virus. This invention also provides a process for preparing a producer cell useful for transferring a foreign gene into a mammalian cell which comprises treating the above-described mammalian cell with a vector plasmid so as to insert the vector plasmid into the cell and thus create the producer cell, the vector plasmid comprising the foreign gene, a functional &psgr; packaging sequence from the helper virus, both the 5′ and 3′ LTRS from the helper virus, and a gene encoding a selectable or identifiable phenotypic trait, and recovering the producer cell so created.