Transformation method for plants
US6372963B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 10, 2000 |
| Grant date | Apr 16, 2002 |
| Priority date | — |
| Expiry date | Jan 10, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for integrating a DNA fragment into the genome of a cell of a monocotyledonous plant, the process comprising the steps of:1) incubating, prior to contacting with the DNA fragment, a culture of untransformed monocotyledonous plant cells on a medium comprising a plant phenolic compound, for a period of time sufficient to stimulate cell division and enhance competence for integration of foreign DNA; and2) contacting the untransformed cells with the DNA fragment under conditions in which the DNA fragment is taken up by the untransformed cells and is stably integrated in the genome of the untransformed cells, to generate transformed cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.