Mixed-bed solid phase and its use in the isolation of nucleic acids

US6376194B2 · kind B2 · utility

Assignee

• Promega Corporation · US

Inventors

• Craig E. Smith · Oregon, US
• Diana L. Holmes · Crystal Lake, US
• Daniel J. Simpson · Middleton, US
• Jehoshua Katzenhendler · Edwardsville, US
• Rex M. Bitner · Cedarburg, US
• Josephine Grosch · Middleton, US

Key dates

• Filing date: Jul 24, 2001
• Grant date: Apr 23, 2002
• Expiry date: Jul 24, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/107497
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Mixed-bed solid phases are provided, with methods for using such solid phases to isolate target nucleic acids, such as plasmid DNA, chromosomal DNA, RNA, or nucleic acids generated by enzymatic amplification from contaminants, including proteins, lipids, cellular debris, or other nucleic acids. The mixed-bed solid phases of this invention are mixtures of at least two different solid phases, each of which has a capacity to bind to the target nucleic acid under different solution conditions, and the capacity to release the nucleic acid under similar elution conditions. By exchanging solution conditions according to the methods of this invention, one can remove contaminants from the target nucleic acid bound to the mixed-bed solid phase, then elute the target nucleic acid in an elution buffer.