Assay for non-peptide agonists to peptide hormone receptors
US6376198B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 12, 1998 |
| Grant date | Apr 23, 2002 |
| Priority date | — |
| Expiry date | May 12, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/72
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention features a method for determining whether a candidate compound is a non-peptide agonist of a peptide hormone receptor. In this method, a candidate compound is exposed to a form of the peptide hormone receptor which has an enhanced ability to amplify the intrinsic activity of a non-peptide agonist. The second messenger signaling activity of the enhanced receptor is measured in the presence of the candidate compound, and compared to the second messenger signaling activity of the enhanced receptor measured in the absence of the candidate compound. A change in second messenger signaling activity indicates that the candidate compound is an agonist. An increase in second messenger signaling activity indicates that the compound is either a full or partial positive agonist; a decrease in second messenger signaling activity indicates that the compound is an inverse (also termed a ‘negative’) agonist.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.