Methods for coexpression of more than one gene using at least one internal ribosome entry site (IRES)
US6376745B1 · kind B1 · utility
Inventors
Key dates
| Filing date | Dec 16, 1999 |
| Grant date | Apr 23, 2002 |
| Priority date | — |
| Expiry date | Dec 16, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8216
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is drawn to a recombinant nucleic acid having (a) a transcriptional promoter; (b) a first plant-expressible structural gene linked to the transcriptional promoter, (c) a cDNA sequence element having an internal ribosome entry site of tobamovirus moving protein (MP) gene (IRESmp) which is located 3′ to the first plant-expressible structural gene, and (d) a second plant-expressible gene located 3′ to the (IRESmp) such that the second gene is placed under the translational control of (IRESmp); wherein the first plant-expressible gene, (IRESmp) and the second plant-expressible gene are transcribed under the action of the transcriptional promoter to give a primary transcript, and the first plant expressible gene of the primary transcript is able to translate by ribosome scanning mechanism and the second plant expressible gene of the primary transcript is capable of translation under the action of (IRESMP). The present invention is further drawn to a method of simultaneously expressing desired genes in vitro and in planta by using crTMV RNA sequences upstream or the MP gene, i.e. IRESmp.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.