Plant-derived map kinase kinase
US6376747B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 27, 1999 |
| Grant date | Apr 23, 2002 |
| Priority date | — |
| Expiry date | Aug 27, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8281
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A mitogen-activated protein (MAP) kinase kinase gene, tMEK2, was isolated from tomato cv. Bonny Best. By mutagenesis, a permanently-active variant, tMEK2MUT, was created. Both wild type tMEK2 and mutant tMEK2MUT were driven by a strong constitutive promoter, tCUP&Dgr;, in a tomato protoplast transient expression system. Pathogenesis-related genes, PR1b1 and PR3, and a wound-inducible gene, ER5, were activated by tMEK2MUT expression revealing the convergence of the signal transduction pathways for pathogen attack and mechanical stress at the level of MAPKK. Activation of biotic and abiotic stress response genes downstream of tMEK2 occurred through divergent pathways involving at least two classes of mitogen-activated protein kinase. This study shows that tMEK2 may play an important role in the interaction of signal transduction pathways that mediate responses to both biotic (eg disease) and abiotic (wound responsiveness) stresses.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.