Starches via modification of expression of starch biosynthetic enzyme genes
US6376749B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 25, 1999 |
| Grant date | Apr 23, 2002 |
| Priority date | — |
| Expiry date | Feb 25, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8245
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch branching enzyme enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of corn comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch branching enzyme structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming corn with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed corn compared to the fine structure of starch derived from corn not possessing said chimeric gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.