PURIFIED CYTOCHROME P450 POLYPEPTIDE CYP76B1 FROM HELIANTHUS TUBEROSUS AND ITS APPLICATIONS AS BIOCATALYST IN PARTICULAR FOR THE DEGRADATION OF ENVIRONMENTAL POLLUTANTS AND FOR ALTERING THE RESISTANCE OF PLANTS SENSITIVE TO PHENYLUREA FAMILY OF HERBICIDES
US6376753B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 30, 1998 |
| Grant date | Apr 23, 2002 |
| Priority date | — |
| Expiry date | Jul 30, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8274
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the gene CYP76B1 of a cytochrome P450 which has been isolated from Helianthus tuberosus tuber. The expression of this gene in yeast has shown that it encoded an enzyme very actively catalysing the O-dealkylation of various exogenous molecules with a high efficiency, such as phenylureas. The expression of CYP76B1 is strongly induced in plants brought into contact with certain exogenous metals or organic compounds. This induction may be exploited for the detection of environmental pollutants. CYP76B1 can be also used to alter the resistance of plants sensitive to this family of herbicides and for soil and groundwater bioremedation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.