Cell-culture and polymer constructs
US6378527B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 24, 1999 |
| Grant date | Apr 30, 2002 |
| Priority date | — |
| Expiry date | Mar 24, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/72
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.