Protein expression strains
US6379924B1 · kind B1 · utility
3Cited by
2References
38Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 15, 1999 |
| Grant date | Apr 30, 2002 |
| Priority date | — |
| Expiry date | Dec 15, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The use of a means to vary Ubc4p or Ubc5p activity in a fungal cell to control the copy number of a plasmid in the cell. The level of Ubc4p or Ubc5p activity may be reduced/abolished (for example by gene deletion, mutagenesis to provide a less active protein, production of antisense mRNA or production of competitive peptides) to raise the copy number and increase yield of a protein encoded by the plasmid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.