Single primer PCR amplification of RNA
US6379932B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 17, 2000 |
| Grant date | Apr 30, 2002 |
| Priority date | — |
| Expiry date | Jul 17, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods and compositions for amplifying RNA sequences by (a) hybridizing to a target RNA a first primer comprising a 3′ target RNA hybridizing sequence and a first 5′ defined amplifyable sequence; (b) extending the first primer with a reverse transcriptase to form a first cDNA strand; (c) hybridizing to the first cDNA strand a second primer comprising a 3′ random cDNA hybridizing sequence and a second 5′ defined amplifyable sequence; (d) extending the second primer with a DNA polymerase to form a second cDNA strand; and (e) amplifying the second cDNA strand with a third primer comprising the first 5′ defined amplifyable sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.