Nucleic acid construct for the cell cycle regulated expression of structural genes

US6380170B1 · kind B1 · utility

Assignee

• Aventis Pharma Deutschland GmbH · DE

Inventors

• Rolf Muller · Marburg, DE
• Ningshu Liu · Berlin, DE
• Jörk Zwicker · Marburg, DE
• Hans-Harald Sedlacek · Marburg, DE

Key dates

• Filing date: Feb 18, 1998
• Grant date: Apr 30, 2002
• Expiry date: Feb 18, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/85
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention refers to a nucleic acid construct comprising at least one activator sequence, at least one chimeric promoter module comprising a nucleotide sequence which binds a protein of the E2F family and a protein of the CDF-1 family, and at least one gene, wherein said chimeric promoter module promotes expression of the gene in the cell cycle later than the B-myb promoter but earlier than the cdc25C promoter. The invention also concerns the purification and identification of CDF-1 protein, and use of this protein to develop new control systems.