Nucleotide compound, nucleotide block oligonucleotide, and method for producing them
US6380378B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 23, 1999 |
| Grant date | Apr 30, 2002 |
| Priority date | — |
| Expiry date | Dec 23, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Novel nucleotide compounds represented by the formula (I) wherein R1 represents a protective group or a PEG bearing organic group; R2, R2′, R2″, R3, R3′ and R3″ each represents a hydrogen atom or an alkyl, cycloalkyl, aryl or aralkyl group which may contain a hetero-atom; B1, B2, B3 and B4 each represents a base, if necessary, protected by a protective group common in nucleotide chemistry or by a PEG bearing organic group; X, X′ and X″ each represents an oxygen atom or a sulfur atom; Y represents an azolyl group, a mono- or di-alkylamino group or a saturated nitrogenous heterocyclic ring; A1, A2, A3 and A4 each represents a hydrogen atom, a hydroxyl group, an alkoxy group or a trialkylsilyloxy group; and m and n each represents 0 or an integer of 1 to 100. The nucleotide of the formula (I) can be used as it is in a reaction mixture for further reaction with 3′-O- and 5′-O-unprotected nucleoside or nucleotide of the formula (II) to yield a nucleotide block or oligonucleotide, and thus is useful as in situ DNA synthesis reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.