Pre-M/M epitopes of dengue virus, synthetic peptides, chimeric proteins and their use
US6383488B1 · kind B1 · utility
Assignees
Inventors
- Susana Vazquez Ramudo
- Guadalupe Guzman Tirado
- Gerardo Enrique Guillen Nieto
- Orlando Luis Pardo Lazo
- Glay Chinea Santiago
- Ana Beatriz Perez Diaz
- Maritza Pupo Antunez
- Rosmari Rodriguez Roche
- Osvaldo Reyes Acosta
- Hilda Elisa Garay Pérez
- Gabriel Padron Palomares
- Maylin Alvarez Vera
- Luis Diaz
- Omaida Perez Insuita
- Jose Luis Pelegrino Martinez De La Cotera
Key dates
| Filing date | Oct 14, 1999 |
| Grant date | May 7, 2002 |
| Priority date | — |
| Expiry date | Oct 14, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.