Methods of labeling nucleic acids for use in array based hybridization assays
US6383749B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 2, 1999 |
| Grant date | May 7, 2002 |
| Priority date | — |
| Expiry date | Dec 2, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and kits are provided for labeling nucleic acids, e.g. for use in array based hybridization assays. In the subject methods, target nucleic acid is generated from an initial nucleic acid source, e.g. mRNA, where the target nucleic acid is characterized by having at least one reactive functionality that is not a moiety found on naturally occurring nucleic acids. Functionalized label is then conjugated to the target nucleic acid, either before or after it has been hybridized to array of nucleic acids stably associated with the surface of a solid support. The subject methods find use in a variety of array based hybridization assays, including differential expression assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.