Methods for producing polypeptides in aspergillus mutant cells
US6383781B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 23, 1999 |
| Grant date | May 7, 2002 |
| Priority date | — |
| Expiry date | Dec 23, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/69
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for producing a polypeptide of interest by (a) cultivating a mutant of a parent Aspergillus cell, wherein (i) the mutant comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of at least one toxin, and (ii) the mutant produces less of the toxin than the parent Aspergillus cell when cultured under the same conditions; and (b) isolating the polypeptide from the culture medium. Also, mutants of Aspergillus cells are provided, as well as methods for obtaining the mutant cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.