Microfabrication of a nuclear transfer array for high-throughput animal cloning
US6383813B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 14, 2000 |
| Grant date | May 7, 2002 |
| Priority date | — |
| Expiry date | Sep 14, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M35/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A micro-machined nuclear transfer array (NTA) provides high-throughput transfer of nuclei between two cells. Donor cells containing the nuclei to be transferred are placed in microwells positioned adjacent to microwells containing the recipient biological cell. The microwells are contained within an upper chamber patterned with parallel rows of microwells of the diameter of the cell of interest. An injection port is formed in the bottom of the microwells through which nuclei may pass during transfer. The NTA also contains a lower chamber having a second array of capture wells of similar dimension and in register with the upper chamber for receiving the nuclei removed from the cell in the upper chamber. Nuclei are removed from the donor cells in the upper chamber, transferred to capture wells in the lower chamber, and inserted into the recipient biological cells of the upper chamber. The upper tray moves relative to the lower chamber so that the nuclei in the capture well are transferred to the recipient biological cell to complete the nuclear transfer process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.