Modified p53 tetramerization domains having hydrophobic amino acid substitutions
US6388062B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 5, 1999 |
| Grant date | May 14, 2002 |
| Priority date | — |
| Expiry date | May 5, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Modified p53 tetramerization domains which do not hetero-oligomerize with native p53 tetramerization domains are described. These modified p53 tetramerization domains have one or more of the following substitutions in the region of residues 325 to 355 of human p53: Leu330 substituted with Phe; Met340 substituted with Phe; Ala347 substituted with Ile; Leu348 substituted with Met; Ala353 substituted with Leu; Gln354 substituted with Leu; Ala355 substituted with Asp. Also described are p53 proteins containing these modified p53 tetramerization domains linked to a p53 DNA binding domain. These proteins and the nucleic acid sequences encoding them, are useful in ameliorating conditions associated with inappropriate p53 function.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.