DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US6388168B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 18, 1998 |
| Grant date | May 14, 2002 |
| Priority date | — |
| Expiry date | Nov 18, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8214
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
DNA constructs are provided for stable transformation of plastids of multicellular plants and expression of foreign proteins in plastids. The DNA constructs comprise a transforming DNA which is targeted to a pre-determined location on the plastid genome and inserted into the plastid genome by homologous recombination with targeting segments comprising DNA sequences homologous to the pre-determined region of the plastid genome. The transforming DNA contains a non-lethal selectable marker gene which confers a selectable phenotype on cells having plastids in which substantially all of the genomes therein contain the transforming DNA (i.e., homoplasmic cells or tissues). The transforming DNA further comprises at least one insertion site 4 for an additional DNA segment, such as a gene encoding a protein for improving a characteristic of the transformed plant. The non-lethal selectable marker gene is preferably provided as a chimeric gene by assembly from an expression cassette comprising 5′ and 3′ regulatory segments, preferably derived from plastid genes. A coding segment encoding the non-lethal selectable marker is inserted between the 5′ and 3′ regulatory segm…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.