Detection of alcaline isophosphatases by electrophoresis
US6391176B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 8, 2001 |
| Grant date | May 21, 2002 |
| Priority date | — |
| Expiry date | Feb 8, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention concerns a process for separating alkaline phosphatase (ALP) isoenzymes from a biological sample by electrophoresis, characterized in that the electrophoresis is carried out on an electrophoresis support after depositing a solution of lectin onto the electrophoresis support in a predetermined localised zone, under conditions which permit interaction between said lectin and the ALP isoenzymes contained in the analysed biological sample, deposition of the lectin solution further being carried out under conditions which are suitable to allow separation of the ALP isoenzymes constituted by the osseous fraction and by the hepatic fraction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.