Monitoring gene expression
US6391549B1 · kind B1 · utility
2Cited by
2References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 1, 1999 |
| Grant date | May 21, 2002 |
| Priority date | — |
| Expiry date | Apr 1, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and system for detecting a parameter of a host cell, a cell culture or medium is provided. A host cell is transfected with an expressible DNA sequence which is under expression control of an inducible promoter sequence. The promoter sequence is inducible in correlation with the parameter, the expressible sequence encoding an enzymatically active product which can catalyze a reaction giving rise to an electrical signal which is detectable in an electrochemical measurement. An electrical signal determined in an electrochemical system then serves as an indication for the parameter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.