Shuttle vectors

US6391582B2 · kind B2 · utility

Assignee

• Rigel Pharmaceuticals, Inc. · US

Inventors

• Ying Luo · Shanghai, CN
• Pei Wen Yu · Burlingame, US
• James Lorens · Otta, NO

Key dates

• Filing date: Dec 9, 1998
• Grant date: May 21, 2002
• Expiry date: Dec 9, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.