Blocker-aided target amplification of nucleic acids
US6391592B1 · kind B1 · utility
33Cited by
2References
28Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 14, 2000 |
| Grant date | May 21, 2002 |
| Priority date | — |
| Expiry date | Dec 14, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention describes a method for blocking an unwanted sequence from been amplified, duplicated or reverse transcribed by using a blocking molecule. Preferred embodiments of the blocking molecule have sequences complimentary at least partially to the unwanted sequence. The preferred blocking molecule can be made of nucleic acids and analogues, for example, peptide nucleic acid and locked nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.