Production of erythropoietin by endogenous gene activation

US6391633B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Anne Stern · Penzberg, DE
• Michael Brandt · Iffeldorf, DE
• Konrad Honold · Penzberg, DE
• Johannes Auer · Schwaigen, DE
• Hans Koll · Oberroth, DE

Key dates

• Filing date: Jan 21, 2000
• Grant date: May 21, 2002
• Expiry date: Jan 21, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/75
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to human cells which are capable, on the basis of an activation of the endogenous human EPO gene, of producing EPO in a sufficient amount and purity to make possible a cost-effective production of human EPO as a pharmaceutical preparation. The invention furthermore relates to a method for the preparation of such human EPO-producing cells, DNA constructs for the activation of the endogenous EPO inhuman cells, and a method for the large technical production of EPO in human cells.