Immunogenic conjugates of Gram-negative bacterial autoinducer molecules

US6395282B1 · kind B1 · utility

Assignee

• University of Rochester · US

Inventors

• Andrew S. Kende · Pittsford, US
• Barbara H. Iglewski · Fairport, US
• Roger Smith · Madison, US
• Richard P. Phipps · Pittsford, US
• James Pearson · Cambridge, US

Key dates

• Filing date: Apr 16, 1999
• Grant date: May 28, 2002
• Expiry date: Apr 16, 2019

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/808
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to an immunogenic conjugate comprising a carrier molecule coupled to an autoinducer of a Gram negative bacteria. The immunogenic conjugate, when combined with a pharmaceutically acceptable carrier, forms a suitable vaccine for mammals to prevent infection by the Gram negative bacteria. The immunogenic conjugate is also used to raise and subsequently isolate antibodies or binding portions thereof which are capable of recognizing and binding to the autoinducer. The antibodies or binding portions thereof are utilized in a method of treating infections, a method of inhibiting autoinducer activity, and in diagnostic assays which detect the presence of autoinducers or autoinducer antagonists in fluid or tissue samples.