Use of phage associated lytic enzymes for the rapid detection of bacterial contaminants
US6395504B1 · kind B1 · utility
24Cited by
27References
38Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 1, 2000 |
| Grant date | May 28, 2002 |
| Priority date | — |
| Expiry date | Nov 23, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/66
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for the use of a phage associated lysing enzyme for the detecting the presence and determining the quantity of bacteria present in or on a wide variety of substances is described. The total concentration of microbes is determined by adding or incorporating a phage associated lytic agent to a disposable test system device with the luminescent reagents luciferin and luciferase, and introducing the disposable test system into a luminometer that can read the luminescence. Other systems can be used with the lytic enzymes for the quantitative and qualitative determination for the presence of bacteria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.