Process for delaying the deactivation of glutaryl amidase during catalysis with a thiol

US6395507B1 · kind B1 · utility

Assignee

• Aventis Pharma Deutschland GmbH · DE

Inventors

• Jürgen Bongs · Wiesbaden, DE
• Johannes Meiwes · Idstein, DE
• Wolfgang Kruse · Hofheim, DE
• Klaus-Peter Koller · Bad Soden, DE

Key dates

• Filing date: May 26, 2000
• Grant date: May 28, 2002
• Expiry date: May 26, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process is provided for delaying the deactivation of glutaryl amidase during catalysis. The amidase is, after maximum conversion of a substrate, separated off by filtration and can be reused in a subsequent reaction batch. In each reaction cycle, the amidase loses activity. The inactivation of the amidase can be delayed by bringing it into contact with a thiol during the reaction. The thiol is preferably 2-mercaptoethanol or cysteine.