Double container device and method for detecting and enumerating microorganisms in a sample
US6395537B1 · kind B1 · utility
Inventors
Key dates
| Filing date | Aug 4, 2001 |
| Grant date | May 28, 2002 |
| Priority date | — |
| Expiry date | Aug 4, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A device for detecting and enumerating microorganisms in a sample substance employs an external container and an internal container inserted into the headspace of the external container. The sample is mixed with liquid growth media in the internal container. Gases generated from the metabolic activity of the microorganisms during incubation expand to the headspace of the sealed external container and interact with an indicator located at a transparent section of the external container. Changes of the indicator are read by an external real time photometer that generates time patterns for analysis. These patterns indicate the presence or absence of microorganisms and assess the level of contamination in the original sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.