Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules
US6396267B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 19, 2000 |
| Grant date | May 28, 2002 |
| Priority date | — |
| Expiry date | Jan 19, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01R33/4608
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for performing polarization transfer in NMR experiments with coupled spin ½ nuclei I and S being irradiated by a sequence of rf pulses comprising a first 90° pulse exciting the spins of the nuclei I and after a delay time a further 90° pulse exciting the spins of the nuclei S is characterized in that there is no inversion pulse acting on the spins of the nuclei S during a time period T between the first 90° pulse exciting the spins of the nuclei I and either the further 90° pulse exciting the spins of the nuclei S or a second 90° pulse acting on the spins of the nuclei I, and that the length of the time period T is chosen such thatd/dT[{square root over (sinh+L (RCT+L )2+L +sin(&pgr;JIST+L )2+L )} exp(−RIT)]is minimized, whereRC is the transverse cross-correlation-relaxation rate of nuclei I,RI is the total transverse relaxation rate of nuclei Iand JIS is the scalar coupling constant between nuclei I and S.This provides a novel polarization transfer element which can be used as a “building block” for a great variety of complex NMR experiments including macromolecules with molecular weights far beyond 100000 and yielding higher s…
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