Patent · US Expired

Method for the enantioselective reduction of 3,5-dioxocarboxylic acids, their salts and their esters

US6399339B1 · kind B1 · utility

181Cited by

1References

16Claims

0Family size

Assignee

Forschungszentrum Jülich GmbH · DE

Inventors

Michael Wolberg · Neutraubling, DE
Michael Muller · Bensberg, DE
Werner Hummel · Titz, DE

Key dates

Filing date	Jul 10, 2001
Grant date	Jun 4, 2002
Priority date	—
Expiry date	Jul 10, 2021

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P41/002
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for the enantioselective reduction of 3,5-dioxocarboxylic acid derivatives and their syntheses. According to the invention, compounds of formula (4) are reacted with an alcohol dehydrogenase which preferably stems from Lactobacillus brevis and which is recombinantly over-expressed in Escherichia coli, in the presence of NADPH. The keto group in position 5 is enantioselectively reduced during this reaction. The keto group in position 3 can also be specifically syn- or anti-reduced in a further step by means of chemical or enzymatic reactions.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.