Use of anti-sense sequences to increase responsiveness to gene amplification
US6399377B1 · kind B1 · utility
Inventor
Key dates
| Filing date | Feb 3, 1999 |
| Grant date | Jun 4, 2002 |
| Priority date | — |
| Expiry date | Feb 3, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/111
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for regulating the production level of a desired protein in a transformed mammalian cell, comprising: a directly amplifiable gene sequence, in expressable form, which when expressed renders said cell more resistant to a toxic agent; a gene of interest, in expressible form, which encodes a desired protein foreign to said cell; and an anti-sense gene sequence, in transcribable form, encoding an anti-sense RNA capable of selectively hybridizing to at least a portion of the mRNA transcribed from said directly amplifiable gene sequence so as to inhibit the translation of said mRNA, such that a higher level of amplification of said directly amplifiable gene and said gene of interest can be achieved, if the cell is exposed to a sufficient level of the toxic agent, that in the absence of said anti-sense RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.