Native-state histoculturing skin extracellular support
US6399380B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 14, 1998 |
| Grant date | Jun 4, 2002 |
| Priority date | — |
| Expiry date | Dec 14, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/54
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for a native-state histoculturing system for skin are disclosed. Skin samples are placed on an extracellular support matrix immersed in a medium whereby the internal surface of the skin is adjacent to the matrix and the external surface is exposed above the surface of the medium and the skin maintained in the medium under skin culturing conditions. An extracellular support matrix comprising a collagen-containing gel and homopolysaccharide sponge or a combination matrix of a top layer of a collagen-containing gel and a bottom layer of a homopolysaccharide sponge is also disclosed. Skin toxicity, hair growth, anti-aging and anti-wrinkling assays utilizing the histoculturing system of the present invention are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.