Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof

US6399861B1 · kind B1 · utility

Assignee

• Dekalb Genetics Corporation · US

Inventors

• Paul C. Anderson · Minneapolis, US
• Christopher E. Flick · Burlington, US
• William J. Gordon-Kamm · Urbandale, US
• Albert P. Kausch · Stonington, US
• Catherine J. Mackey · Old Lyme, US
• Emil Orozco · Cochranville, US
• Peter M. Orr · Pawcatuck, US
• Michael A. Stephens · East Lyme, US
• David Walters · Bloomington, US
• Donald S. Walters · Mystic, US

Key dates

• Filing date: May 23, 1995
• Grant date: Jun 4, 2002
• Expiry date: May 23, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/87
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.