Process for intercalation of spacer molecules between substrates and active biomolecules
US6402899B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 27, 2000 |
| Grant date | Jun 11, 2002 |
| Priority date | — |
| Expiry date | Jan 27, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A wide variety of substrates can be functionalized to attach spacer molecules therein by exposing the substrates to a cold plasma ignited in dichlorosilane, silicon tetrachloride or hexachlorodisilane gas to implant silicon-chlorine functionalities in the substrate surface. The plasma implanted surface functionalities can then be utilized to initiate second stage gas phase derivatization reactions to form linker molecules attached to the substrate. Active biomolecules such as enzymes can then be bound to the exposed linker molecules to bind the bioactive molecules to the substrate while allowing freedom of movement and conformation of the bound molecule comparable to that of the free molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.