Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays
US6403309B1 · kind B1 · utility
35Cited by
15References
37Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 19, 1999 |
| Grant date | Jun 11, 2002 |
| Priority date | — |
| Expiry date | Mar 19, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to methods and compositions for use in screening nucleic acid populations for nucleic acid polymorphisms. The methods, referred to generally as ValiGeneSM Mutation Screening, Peptide-Linked (VGMS-PL) methods, are specifically designed for high-throughput genotype mapping and gene expression analysis of animal and plant nucleic acids without requiring a PCR amplification step. In particular, the methods of the invention utilize oligonucleotide probes labeled with distinguishable and identifiable peptide tags, that are captured on addressable antibody arrays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.