Mutant proteolytic enzymes and method of production
US6403331B1 · kind B1 · utility
6Cited by
4References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 31, 2000 |
| Grant date | Jun 11, 2002 |
| Priority date | — |
| Expiry date | May 31, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B20/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Mutant Bacillus lentus DSM 5483 proteases are derived by the replacement of at least one amino acid residue of the mature form of the B. lentus DSM 5483 alkaline protease. The mutant proteases are expressed by genes which are mutated by site-specific mutagenesis. The amino acid sites selected for replacement are identified by means of a computer based method which compares the three dimensional structure of the wild-type protease and a reference protease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.